Search results for " binding"
Article
How To Interpret Protein Binding Capacity: Chromatography Resins
Explore what protein binding capacity is, how it can be measured and what exactly to look for on a manufacturer’s specification sheet in this blog post.
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Article
Determining and Optimizing Dynamic Binding Capacity
Dynamic binding capacity (DBC) indicates the quantity of a specific biomolecule that can be bound during a chromatography purification step under a specific set of process conditions. Determ…
Article
Dynamic Binding Capacity in Fiber-Based Chromatography
Just like with resins, the dynamic binding capacity (DBC) of a Fibro unit is affected by process conditions and by the properties of the target molecule. Thus, determining the DBC of a Fibro unit is s…
Article
Alkaline stability of Fc and VH3 binding in protein A resins
In this study, we have looked at how different antibody binding sites of a protein A ligand are affected by cleaning in place (CIP). We evaluated NaOH incubation and the effect of performed CIP cycles…
Article
How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
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In protein purification, dynamic binding capacity (DBC) of a chromatography column describes the maximum amount of target protein that you can load onto your co…
Article
Interpreting Protein Binding Capacities for Chromatography Resins
In this post, we explain what protein binding capacity is, how it can be measured, and what to look for on a manufacturer's specification sheet.
Poster
Optimizing dynamic binding capacity and aggregate clearance in an mAb polishing step
In this study, optimization of binding conditions targeting high dynamic binding
capacity (DBC) and aggregate clearance was performed.
Article
Bind, Cut, Elute — Easy Affinity Tag and Protease Removal
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This blog post discusses automated methods for removing an affinity tag and the protease. The tag is cleaved while the protein is still bound to the affinity re…
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
Compared to traditional small-molecule drugs, their structures are complex, and they may assert their effects by binding to more than one target molecule. To date, the majority of monoclonal antibodie…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
SPR is a rapidly emerging tool for studying ligand binding interactions with membrane proteins, which are the major molecular targets for biopharmaceutical products (6). In SPR, one of the interactant…